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Aynsley Hartney has a diverse work experience that spans multiple industries. In 2017, they worked as a Research and Development Intern at Medtronic Spine & Biologics, where they engineered surgical instruments and improved existing models. Aynsley continued their research work as a Clinical Researcher at Greenville Health System from 2017 to 2018, where they analyzed the effect of physician consultation on patient length of stay and quality of care. During this time, they also presented their findings at the Annual Industrial and Systems Engineering Conference. Aynsley then joined Clemson University in 2018 as an Undergraduate Research Assistant, providing support in research projects until the end of 2018. In 2019, they participated in the Pediatric Oncology Education Program at St. Jude Children's Research Hospital. Later that year, they became an Undergraduate Student Researcher in the Department of Public Health Sciences at Clemson University until the end of 2020. In 2021, Aynsley expanded their role as they worked as an Intern at Church Health and a College Essay Tutor at Tutor.com.

Aynsley Hartney completed their education with a Doctor of Medicine (MD) degree from the University of Wisconsin-Madison, where they attended from 2021 to 2025. Prior to that, they obtained a Bachelor of Science (BS) in Bioengineering with a concentration in Biomaterials from Clemson University. Aynsley attended Clemson from 2016 to 2020. Additionally, Aynsley participated in a study abroad program at DIS, focusing on Human Health and Disease. No specific years are provided for this program. Lastly, they pursued Irish Studies and Statistics at University College Dublin, although no start or end year information is given for this educational experience.