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Guanglei Cui has extensive work experience in various scientific roles. Guanglei is currently serving as the Director of Computational Design at Treeline Biosciences since April 2021. Prior to this, they worked at GlaxoSmithKline Pharma GmbH for five years, from 2016 to 2021, where they held the position of Senior Scientific Investigator and GSK Associate Fellow. Guanglei also worked as an Investigator at GlaxoSmithKline Pharmaceuticals from 2011 to 2016. Guanglei Cui's earlier experience includes working as a Principal Scientist at GlaxoSmithKline Pharmaceuticals from 2007 to 2010. Guanglei started their career as a Postdoctoral Research Associate at the University of Florida from 2005 to 2007 and at Pensylvannia State University from 2004 to 2005.

Guanglei Cui obtained a Bachelor's degree in Applied Chemistry from the University of Science and Technology of China, where they studied from 1990 to 1995. Guanglei then pursued a Master's degree in Polymer Chemistry at The Chemistry Institute, the Academy of Sciences of China, from 1995 to 1998. Following this, Guanglei Cui attended Stony Brook University from 1998 to 2003, where they obtained a Ph.D. in Computational Chemistry.