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Luke Denison has a diverse work experience, beginning with a role as a Host at WTIU-TV (PBS) in 2013. That same year, they also worked as a Research Intern at Sarah Cannon Research Institute. In 2015, they were a Research Assistant at Vanderbilt University Medical Center. From 2016 to 2020, they held various roles at CITYMD, including Account Manager - Occupational Medicine and Corporate Relations, Assistant Manager, and Medical Scribe. Most recently, they have been a Partnership Manager, Healthcare Providers at SIRUM since 2021, and prior to that, they were the Director of Business Development and Sales and Senior Account Manager, Customer Success at Juxly.

Luke Denison earned a Bachelor's degree in Theatre/Theater and Chemistry from Indiana University Bloomington.