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Anne Fleming has a diverse work experience in the veterinary field. Anne began their career at Spinnaker Veterinary Clinic as an Associate Veterinarian in June 2013 and stayed with the clinic until April 2017. Anne then moved on to work at Countryside Veterinary Clinic as an Associate Veterinarian from April 2017 to September 2017. Following that, they briefly worked at The Family Pet Hospital as an Associate Veterinarian for three months from September 2017 to December 2017. Anne then returned to Spinnaker Veterinary Clinic in December 2017 and continued as a Veterinarian until January 2024. Their most recent role is as a Medical Director at PetVet Care Centers since July 2023.

Anne Fleming has a diverse education history. Anne obtained a Bachelor of Arts in Economics and Russian Language and Literature from Dartmouth College in 1987. Anne then pursued a Master of Business Administration with a focus on Marketing from Duke University - The Fuqua School of Business, graduating in 1992. In 2009, Anne earned a Doctor of Veterinary Medicine degree from Tufts Cummings School of Veterinary Medicine. Additionally, they completed a Master of Public Health degree from Tufts University in 2013.