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Jack Walker has held various roles in the healthcare, laboratory, horse racing and veterinary industries. In 2022, they began working as a Technical Product Specialist for NoBACZ Healthcare. From 2020 to 2021, they worked as a Laboratory Technician for SYNLAB UK & Ireland, where they ran analysers in biochemistry, endocrinology and haematology, prepared and read urine sediments, and organized the postage of biohazard specimens. From 2020 to 2021, they were a Yard Supervisor for The National Stud- Part of The Jockey Club. From 2019 to 2020, they worked as a Stud Groom for Cambridge Stud Farm, preparing 56 yearlings for the Karaka 2020 sales and being part of the 'on call' foaling team. From 2019 to 2020, they were also a Student for The National Stud- Part of The Jockey Club. From 2014 to 2020, they worked as a Veterinary Assistant for Hird and partners.

Jack Walker attended Greenhead College from 2013 to 2015 and then went on to the University of Hull to pursue a degree in Biological Sciences, with a focus on Animal Ecology and Genetics, from 2015 to 2018.