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Rachana Saini has worked in a variety of roles over the past five years. In 2017, they worked as a Summer Internship at Amul India. In 2018, they were a Research Intern at CSIR-Institute of Himalayan Bioresource Technology (IHBT). In 2020, they were a Research Intern at the National Institute of Plant Genome Research. In 2021, they were a Research Analyst at Desam Organics. Currently, they are a Quality Research Analyst at NAT HABIT.

Rachana Saini completed their M.tech in Biotechnology from Banasthali Vidyapith in 2021, and their B.tech in Biotechnology from the same university in 2019.