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Antonio Choi has a strong background in healthcare management. Antonio started their career in 2009 as a Nurse Manager at Shepherd Lodge, where they worked until 2017. Antonio then moved on to St. Joseph's Health Centre, Toronto, where they served as a Patient Care Manager for six years. Currently, Antonio is employed as the Assistant Director of Resident Care at the Mon Sheong Foundation since 2023.

Antonio Choi's education history begins in 1986 when they attended The Hong Kong Hospital Services Department School of General Nursing to study General Nursing. Antonio completed their studies in 1989. In 1992, they enrolled at the University of Western Sydney, Nepean and obtained a Bachelor of Health Science (Nursing) degree in 1993. Antonio continued their education in the field of nursing, attending the Canadian Nurses Association from 2002 to 2007 and the Emergency Nurses Association from 2002 to 2006 and again from 2001 to 2005. In 1998, they attended Centennial College, and in 1999, they pursued further education at the same institution. Following these experiences, Antonio completed their education at Queen's University in 2008, although the degree and field of study are not specified.