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Subuddhi Kulkarni has a diverse work experience in the field of pharmacy spanning over two decades. They have held various roles such as Lecturer in Pharmacy at Shri Govindram Seksaria Institute of Technology & Science and College of Pharmacy, IPS Academy. Additionally, they have worked as a Staff Pharmacist, Pharmacy Manager, and Director at companies like Loblaw Companies Limited, Shoppers Drug Mart, and Hotel Dieu Shaver Health and Rehabilitation Centre. Their most recent role was as Director, Pharmacy, and Quality Improvement at Hotel Dieu Shaver Health and Rehabilitation Centre.

Subuddhi Kulkarni completed a Bachelor of Pharmacy at Devi Ahilya Vishwavidyalaya from 1992 to 1996. Subuddhi then pursued a Master of Pharmacy in Pharmaceutics at Savitribai Phule Pune University from 1996 to 1998, as well as a bridging program for international pharmacy graduates at the University of Toronto in 2003 and 2004. Additionally, they earned a Master of Business Administration in Innovation Leadership from the University of Fredericton from 2015 to 2017. Subuddhi also holds certifications in Essentials of Oncology for Pharmacists, Certified Diabetes Educator, Injection and Immunization certification, and Opioid Dependence and Treatment Program.