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N.S. Balasubramanian started their work experience as the General Manager at United Pharmacies in January 1994. N.S. worked in this role until August 2004. In September 2004, they joined Gulf Marketing Group as the President Healthcare Division, where they were also a Member of the Executive Committee. N.S. held this position until February 2019. In February 2019, Balasubramanian became the Chief Executive Officer of Aster Pharmacy.

N.S. Balasubramanian attended Dr. MGR Medical University from 1989 to 1993. During this time, they earned a Bachelor of Pharmacy (BPharm) degree in the field of Pharmacy.