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Mark Trenholm has a diverse work experience in conservation and environmental management. Mark started their career as the Executive Director at Tillamook Estuaries Partnership in 2000, where they served for eight years. In 2008, they joined the Wild Salmon Center as a Senior Program Manager, overseeing various conservation projects. Over the years, they progressed to the role of Coastal Program Director, responsible for directing programs focused on coastal ecosystems. Trenholm's most recent position is as the Vice President of Conservation at the Wild Salmon Center, a role they have held since January 2023.

Mark Trenholm attended the University of Oregon from 1997 to 1999. Mark did not receive a degree or specialize in a specific field of study during their time at the university. Prior to that, from 1989 to 1993, Mark attended the University of North Carolina at Charlotte. Similar to their time at the University of Oregon, they did not obtain a degree or focus on a specific field of study at this university either.