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Akie Hayashi has a diverse range of work experience in the nursing field. Akie started their career as a Perioperative Nurse at Northern Health in 2015. Akie then moved to Vision Eye Institute in 2016 where they held multiple roles including Perioperative Nurse, Clinical Nurse Specialist, and Associate Nurse Unit Manager. Currently, they are serving as the Director of Nursing at the same institute. Akie Hayashi's most recent position began in May 2020.

Akie Hayashi completed a Bachelor of Nursing with a focus on Perioperative/Operating Room and Surgical Nursing at Flinders University from 2012 to 2013. In addition to their formal education, they also obtained certifications in "Leading Yourself" and "Learn Emotional Intelligence, the Key Determiner of Success" from LinkedIn in September 2021. They also obtained a certification in "Teamwork Foundations" from LinkedIn in 2015.