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Yvonne Ornelas-Rios began their work experience in 2001 as a Clinical Instructor at the University of Kansas School of Nursing. In 2013, they transitioned to become a Nursing Educator at Kansas City Kansas Community College, a role they held until 2015. In 2018, they joined Vibrant Health - Wyandotte Neighborhood Clinics as a Primary Care Provider and Family Nurse Practitioner. Yvonne continues to work in this position.

Yvonne Ornelas-Rios obtained a Master's Degree in Advance Practice Nursing from the University of Kansas School of Nursing, which was completed in 1999.