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Asimina Touloupa began their work experience in 2008 at the Hellenic Statistical Authority (ELSTAT) as a Statistical Assistant, where they worked until March 2011. In 2013, they worked as a Finance Intern at Johnson & Johnson for a period of two months. Later that same year, they started working as an Accounting Associate at Speed Couriers, where they stayed until September 2014. In 2015, they joined PwC Greece as a Tax Associate and worked there until October 2018. From October 2018 to April 2021, they held the position of Senior Tax Associate at PwC Greece. Currently, they are employed as a Senior Accountant at Uni Systems, starting in April 2021.

Asimina Touloupa completed their education at the Athens University of Economics and Business from 2008 to 2013. Asimina obtained a Bachelor's Degree in Business Administration, Management and Operations during this time. Before attending university, they attended the 1st High School of Ymittos from 2005 to 2008, where they achieved an 18.6 Distinction, although the specific field of study is not mentioned.