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Rotem Tidhar has worked in a variety of roles since 2002. Beginning in 2002, Rotem Tidhar worked as a Charter department for Tashoot Yacht Sales & Charters. From 2010 to 2015, Rotem Tidhar was a PhD Student at the Weizmann Institute of Science. Afterward, Rotem Tidhar was a Postdoctoral Fellow at the Weizmann Institute of Science from 2015 to 2017. In 2017, Rotem Tidhar became the Lab Manager at Hutchison Kinrot. Finally, since 2016, Rotem Tidhar has been the VP R&D and Research and Development Manager at TripleW Ltd.

Rotem Tidhar began their educational journey in 2005 when they obtained a B.Sc. in Biology from Ben-Gurion University of the Negev. From 2007 to 2014, they attended the Weizmann Institute of Science, where they studied Biochemistry and Molecular Biology.