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Manuel Holstein has worked at various companies since 2008. Manuel began their career as a Production Assistant at Show Off Filmes in 2008. In 2009, they became a Psychologist, Team Leader and Project Coordinator at Centro Social e Paroquial de Alcântara. In 2011, they worked as a Sales and Services representative at Barclaycard. From 2012 to 2017, they worked at Allianz Worldwide Care in various roles, including Senior Technical Specialist, Technical Specialist, Senior Claims Officer and Claims Officer. In 2017, they became a Business Process Operations Manager at UnitedHealth Group. In 2018, they worked as a Senior Business Process Analyst at OutSystems. In 2020, they became the President of Apmobi - Associação Portuguesa para a Mobilidade. In 2021, they were the Co-Founder & CEO at Sense Health and the Head Of Operations Portugal at Tiko.

Manuel Holstein is currently enrolled in ThePower Business School, where they began their studies in 2020. Manuel previously obtained an MBA from Universidade Lusófona de Humanidades e Tecnologias in 2011, and a Master of Science in Applied Psychology from Instituto Superior de Psicologia Aplicada in 2005. In addition to their formal education, Manuel has obtained several certifications, including a Prince2 Practitioner from BCS, The Chartered Institute for IT in 2017, an APA Private Medical Insurance from the Insurance Institute of Ireland in 2013, and a Certification of Advanced English, CAE from the University of Cambridge in 2012.