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Icaro Souza began their work experience in 2017 as a Lighting Technician at Gloucester Stage Company. From February to April 2019, they had an internship at Cogen Immune Medicine. In July 2020, Icaro joined Synlogic, Inc. as a Laboratory Technician.

Icaro Souza attended Gloucester Biotechnology Academy from 2018 to 2019, where they obtained a Biotechnology certificate with a focus on Biotechnology Laboratory Technician. During the same period, they also studied at Gloucester Genomics Marine Institute, although no specific degree or field of study is mentioned for this institution.