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Francis-Xavier Barretto started their work experience as a Trainee Microbiologist at Laboratories Griffon Ltd. in 2006. In 2007, they joined Swati Spentose Pvt. Ltd. as an Innovative Projects Manager. Later on, they became the Chief Scientific Officer and then the Director of Business Development at the same company. Francis-Xavier held these roles until the present, without specific end dates provided.

Francis-Xavier Barretto completed their education in the field of Microbiology and Biochemistry. From 1999 to 2004, they attended St. Xavier's College, where they earned a Bachelor of Science degree. Following this, from 2004 to 2006, they enrolled at the Institute of Science in Mumbai and obtained a Master of Science degree in Microbiology.