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Huisun Lee has worked in a variety of roles since 2000. From 2000 to 2001, they were a Strategy Planning Team Member for the Electronic Chemical Material Division of Samsung Cheil Industry. From 2001 to 2006, they were an Associate Research Engineer at Asan Medical Center (AMC). From 2006 to 2008, they were a Research Professor at Sookmyung Women's University. From 2008 to 2010, they were a Postdoctoral Researcher at the University of Ulsan. From 2010 to 2011, they were a Postdoctoral Researcher at the University of Michigan. From 2011 to 2016, they were an Assistant Research Professor and Postdoctoral Researcher at the University of Kansas. From 2016 to 2019, they were a Research Scientist at Lehigh University. Since 2021, they have been a Principal Scientist at Standigm.

Huisun Lee completed their Bachelor's degree in Polymer Chemistry from Dankook University between 1992 and 1996. Huisun then went on to obtain their Master's degree in Polymer Materials from Seoul National University between 1998 and 2000. Finally, they earned their Doctor of Philosophy (Ph.D.) in Computational Biology from the University of Ulsan between 2003 and 2006.