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Vihasi Jani has worked in a variety of roles in the medical research field. In 2015, they began their career as a Graduate Research Assistant at Prabhu lab, Department of Veterinary and Biomedical Sciences. In 2016, they were an Intern at the Naval Medical Research Center, followed by a Research Assistant, Research Associate, and Research Associate II at The Henry M. Jackson Foundation for the Advancement of Military Medicine from 2017-2022. Currently, they are a Research Associate II at Spotlight Therapeutics.

Vihasi Jani obtained their Bachelor of Pharmacy (B.Pharm.) from Nirma University, Ahmedabad, Gujarat, India between 2011 and 2015. Vihasi then went on to pursue a Master's degree in Biotechnology from Penn State University between 2015 and 2016.