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Diane Serena's work experience includes various roles in the healthcare industry. Diane started their career as a Ward Secretary at Ely Bloomenson Community Hospital and Nursing Home in 2003 and worked there until 2005. From 2005 to 2007, they worked as a Licensed Practical Nurse at St. Mary's Duluth Clinic. In 2007, they became the Registered Nurse Manager at Boundary Waters Care Center and held the position until 2012. In the same year, they joined Spectrum Health as an RN Facility Manager and later became the NE Regional Executive Director at Spectrum Health Companies in 2012. Since 2018, they have been working as an RN Care Coordinator at Essentia Health. Additionally, they also owned and operated Serena's Carpet Shop from 1981 to 2003 and continues to provide consultation as needed.

Diane Serena has an Associate Degree in Nursing, which they obtained from Excelsior College between the years 2005 and 2007. Prior to that, they completed their LPN (Licensed Practical Nurse) certification at Moorhead State University in 2004. In 1975, Diane also acquired an LPN certification from Eveleth Technical School. It is worth noting that there is no information available regarding any additional certifications they may have obtained.