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Trupti Gaikwad has a diverse work experience across various industries. Trupti currently serves as the Director of Advocacy and Policy at Santhigram Foundation, a non-profit organization. Prior to this, they held the position of Director of Operations at Santhigram Wellness Kerala Ayurveda, where they also worked as an Ayurvedic Practitioner and Centre Head. Trupti has also worked as a Fitness Coach at LA Fitness and as an Instructor at JRS College of Business and Healthcare Inc. Additionally, they have experience as a Medical Administrator at Dr Bream & Associates and as a Medical Officer at National Rural Health Mission.

Trupti Gaikwad holds a Bachelor's Degree in Ayurvedic Medicine/Ayurveda from S.S.R.A Medical College & Hospital, completed in 2011. In 2015-2016, they earned a Postgraduate Diploma in Workplace Wellness and Health Promotion from Centennial College. This was followed by a Post Graduate Diploma in Health/Health Care Administration/Management from St. Lawrence College, completed in 2016-2017. Trupti also pursued Post Graduate studies in Mother and Child Wellness at the National Institute of Ayurveda, Jaipur. In terms of additional certifications, they obtained the Certified Personal Fitness Trainer - Health Fitness Instructor (CPFT-) credential from NFPT--The National Federation of Professional Trainers in 2023.