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Ashley Callow has a diverse work experience in human resources and management. Ashley started their career as a Store Manager at The Buckle in 2009. In 2010, they joined Army Civilian Service as a Human Resource Specialist, a role they held for two years before becoming a Senior Human Resources Business Partner in 2016. In the same year, they joined Saint Luke's Health System as a Senior Human Resources Business Partner and was later promoted to the position of Director of Human Resources in 2019.

Ashley Callow holds a Master of Business Administration (MBA) degree from St. Ambrose University, which they obtained from 2011 to 2012. Prior to that, they completed their undergraduate studies at the University of Missouri-Columbia from 2003 to 2007, majoring in Human and Environmental Sciences with a focus on Apparel and Textile Management. No additional information is provided about their education history.