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Thu Do has been working in the field since 2010, starting as a Math and Science Tutor at Evergreen Valley College. In 2013, they became a Pharmacy Clerk at Rite Aid Pharmacies. In 2015, they took on the role of Medical Receptionist and Billing Assistant at Tully Health Center. In 2016, they began working at Thermo Fisher Scientific, taking on roles such as Manufacturing Scientist, Senior Resin Qualification Technician, and Resin Qualification Technician. In 2022, they began working as a Sr Research Associate II at ReCode Therapeutics.

Thu Do obtained an Associate of Arts - AA in Chemistry from Evergreen Valley College in 2009-2012. Subsequently, Thu Do completed a Bachelor's degree in Pharmaceutical Chemistry from the University of California, Davis in 2012-2014.