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Ai Lee Ng has held various roles throughout their work experience. Ai Lee started their career as a Senior Consultant at Biaxident Paradigm Sdn. Bhd. (Global MSDS Ltd) in February 2011 and remained in this role until June 2018. Ai Lee then joined Dymax Corporation as the Regional Regulatory & Compliance Manager for Asia in August 2018, where they worked until April 2020. Most recently, Ai Lee Ng has been working at Lisam Systems, starting as a Regulatory Affairs & Business Consultant in April 2020. Ai Lee later took on the role of Lisam Singapore Director in July 2021. The end dates for their current roles at Lisam Systems are not provided.

Ai Lee Ng attended Universiti Tunku Abdul Rahman (UTAR) from 2007 to 2010, where they earned a Bachelor of Science (BS) degree in Biochemistry. In addition to their degree, they have obtained certifications in "Manage Hazardous Substances" from Workforce Singapore in April 2019 and "Dangerous Goods" from IATA in September 2019.