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Persephone Poulton has a diverse work experience that spans various roles and industries. Starting in 2012, they gained work experience as an Assistant Electrical Engineer at SSE Renewables, where they assisted with testing machinery and communicating with the public. Persephone then worked as a Waitress and Front of House at Havly Café, where they focused on customer satisfaction and performed various tasks. In 2015, Persephone became a Notetaker at Randstad Opus, and in 2016, they worked as a Summer Research Student at KP Technology. Persephone also worked as a Waitress (Seasonal) at Da Steakhoose and as a Retail Assistant at Jannettas Gelateria. From 2013 to 2018, Persephone was an Undergraduate Student at the University of St Andrews, where they also had a Summer Internship involving the development of an Arduino-based teaching tool. Persephone continued their education at the university as a Researcher PhD Student from 2018 to 2022. Persephone's most recent role is as a Product Development Engineer at PowerPhotonic, starting in 2022.

Persephone Poulton completed their high school education at Anderson High School from 2007 to 2013. Persephone then pursued a degree in Experimental Physics (MPhys) at the University of St Andrews, which they completed from 2013 to 2018. In addition to their academic achievements, Persephone has also attained certifications in Astrobiology from the Astrobiology Summer Academy, a Driving Licence from the Driver and Vehicle Licensing Agency (DVLA), a Grade 4 Piano from ABRSM, and a Rainbow Dancing Award from Anglia Regional Theatre School. No specific details about the obtained month or year for these certifications were provided.