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Diana Carolina Ballesteros has worked in several roles since 2007. Diana Carolina began as a tissue culture worker at the Universidad Nacional de Colombia in 2007. In 2010, they began working as a Graduate Student at the University of Arkansas. From 2014 to 2018, they were a Research Associate at Driscoll's, and then moved on to Pebble Labs Inc. where they held the roles of Research Technician, Research Associate, and Senior Research Associate.

Diana Carolina Ballesteros obtained a Bachelor's degree in Agronomy and Crop Science from Universidad Nacional de Colombia between 2004 and 2009. Diana Carolina then went on to pursue a Master's degree in Agronomy and Crop Science at the University of Arkansas, which they completed in 2013.