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Mary Charlton has a significant amount of work experience in the pharmaceutical and clinical research industry. Mary started their career at Thomas Jefferson University Hospital as a Clinical Research Coordinator in the Radiology Department. Mary then joined ERT as a Project Manager in the Diagnostics Division before moving to Wyeth as a Senior Project Planner. After that, Mary worked at Centocor as a Clinical Project Planner and bioMerieux as a Project Manager/Consultant. Their longest tenure was at Parexel, where they held various roles including Senior Project Leader, Principal Lead Super User for eClinical Solutions, Associate Project Director for Corporate Initiatives, Scientific Data Solutions Architect and Program Director, and currently, they are an Associate Director for Business Process Excellence.

Mary Charlton attended the University of the Incarnate Word from 1988 to 1993, where they obtained a Bachelor of Science degree in Nutrition.