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Jayne Respeliers has worked in various roles since 2014. Jayne began their career as a Temporary Youth Specialist for Cedars Youth Services. In 2016, they became a Manager at Athleta, where they were the Customer Experience Lead. In 2018, they became a Yoga Instructor at Genesis Health Clubs. From 2019 to 2022, Jayne worked as an Account Manager at Markel, Foresight Commercial Insurance, and Pathpoint.

Jayne Respeliers attended the University of Nebraska-Lincoln from 2013 to 2016, where they earned a Bachelor of Education and Human Services. Her field of study was Family Science, Pyschology, and Sociology. In 2014, they obtained a CPR certification from the Red Cross Blood Services.