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Leslie Au began their career in 2005 as a Graduate Student at the University of Washington. In 2008, they moved to Washington University in St. Louis to pursue a PhD, and in 2009 they joined Oceanit as a Senior Biomedical Engineer.

Leslie Au obtained a Ph.D. in Biomedical Engineering from Washington University in St. Louis in 2009. Prior to that, they obtained a Master's degree in Chemistry from the University of Washington in 2008. Leslie also obtained a Bachelor of Science degree in Cell and Molecular Biology, Chemistry and Biochemistry (ACS certified) from the University of Washington in 2005. Her educational history began at Pearl City High School.