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Madhavee Buddhikot has a diverse work experience, starting with their role as a Postdoctoral fellow at UCSF from 2000 to 2002. Madhavee then worked as a Post Doctoral at the Children's Hospital Oakland Research Institute from 2002 to 2005. Following this, Madhavee joined BioRad as a Sr. Technical Support Consultant, providing support for Bio-Rad's chromatography and Bio-Plex products from 2005 to 2006. In 2006, they joined Molecular Devices as an Application Scientist, where they worked until 2012. During their time at Molecular Devices, Madhavee also served as a Technical Support Team Leader for Bioresearch, leading a team and ensuring customer satisfaction. From 2012 to 2015, Madhavee worked as a Technical Support Leader in the field of Drug Discovery. In 2016, they were promoted to the position of Global Operations Manager, Advanced Workflow Engineering Solutions (AWES) at Molecular Devices, where they were responsible for overseeing operations of paid custom projects and ensuring revenue growth and on-time delivery. Currently, they hold the role of Senior Global Operations Manager, Automation & Workflow Solutions Actions at Molecular Devices. Additionally, Madhavee has also served as the Executive Director of Green Kids Now, Inc from 2010 to 2016.

Madhavee Buddhikot completed an Advanced Certificate in Regulatory Affairs at San Diego State University. Madhavee also obtained a Ph.D. in Biochemistry from NIRRH, University of Mumbai. In addition, they completed a Core:Credential Readiness program at Harvard Business School Online.