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Jason Lyle has a diverse work experience spanning over 20 years. Jason started their career as an Experimental Officer at Covance in 2000 and then moved on to work as a Research Assistant at the University of Bradford in 2002. After that, they worked as a Scientist at Cyprotex Discovery from 2004 to 2007. In 2008, they joined Covance again, this time as a Medical Writer, until 2009. From 2009 to 2011, they worked as a Bioanalytical Researcher at the University of Bradford. In 2011, they joined Thermo Fisher Scientific as an Area Account Manager and stayed with the company until 2016. In the same year, they worked as a Regional Marketing Manager at CAS for a year. Jason then moved on to Waters Corporation, where they served as an Account Manager from 2017 to 2019. In 2019, they joined Intertek as a Business Development Manager until 2020, when they became an Account Manager at Molecular Devices.

From 1997 to 2000, Jason Lyle attended Queen's University Belfast, where they pursued a Bachelor of Science (BSc) degree in Biochemistry.