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Tsuyoshi Okuno has a diverse work experience spanning several years. Tsuyoshi began their career at Mitsubishi Tanabe Pharma Corporation in 2002 as a Researcher and held various roles within the company until 2019, including Project Manager, Manager of Product Strategy, and Senior Manager of Corporate Strategy. In 2019, Okuno joined MP Healthcare Venture Management Inc. as a Director until 2023. Currently, they serve as the Director of the Neurology Group in the Global Product Strategy Department at Mitsubishi Tanabe Pharma Corporation.

Tsuyoshi Okuno earned a Bachelor's degree in Pharmacy, Pharmaceutical Sciences from Osaka University in 2000. Tsuyoshi then went on to obtain a Master's degree in the same field from Osaka University in 2002. In 2006, Okuno pursued a Doctor of Philosophy degree in Veterinary Sciences/Veterinary Clinical Sciences, General from Osaka Prefecture University, which they completed in 2010. Later, from 2015 to 2017, they pursued a Master of Business Administration - MBA degree with a specialization in Management Accounting from Osaka Prefecture University.