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Pit Janssen has a diverse work experience spanning various roles and industries. Pit co-founded MedAppNL in 2015, which provides pharmacy services through a mobile app. In the same year, they also co-founded Wolfpack IT, a software development company. Prior to that, in 2014, Pit founded JP Medical & Cosmetics, aiming to bring therapeutic cosmetic brands to Western Europe. Pit also worked as an interim pharmacist and owner at PharmP from 2014 to 2015. Before their entrepreneurial ventures, Pit worked as a student assistant at Universiteit Utrecht from 2010 to 2014 and as a scientific employee at Diemer Apotheken from 2009 to 2013, where they delivered scientific presentations. Additionally, Pit gained experience through internships at Mediq, GlaxoSmithKline, and McMaster University.

Pit Janssen completed their Bachelor of Science (BSc) in Pharmaceutical Sciences at Utrecht University from 2007 to 2010. Pit then pursued further education at the same university, graduating with a Doctor of Pharmacy (PharmD) degree in Pharmaceutical Sciences from 2010 to 2013.