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Ashley Holmes has a diverse work experience spanning multiple industries. In 2007, they worked as a Media and Promotions Intern at AKA UK. The following year, they were a Production & Entertainment Assistant at Barkley Kalpak Associates. In 2009, Ashley joined Manhattan Eyeworks as an Office Manager, where they stayed until 2017. At Manhattan Eyeworks, they held multiple roles, including Head of Marketing starting in February 2022 and Marketing Manager since April 2017.

Ashley Holmes obtained a Bachelor's Degree in Theatre Arts Management from Ithaca College, which they attended from 2005 to 2008. Additionally, they have obtained several certifications in various fields including motion graphics, basic After Effects training, expressions in After Effects, advanced formulas and functions in Excel, exploring and describing data fluency, essential Excel training, and data analytics. These certifications were obtained through LinkedIn in the months of July, August, and September 2020.