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Mark Pavlyukovskyy has held a variety of roles in the past 14 years. In 2007, they were a Congressional Page for the U.S. House of Representatives. From 2010 to 2012, they were a Molecular Biologist in the Coller Lab and an Adel Mahmoud Global Health Scholar at Princeton University, and a Neuroscience Researcher at the Howard Hughes Medical Institute. In 2011, they were a Research Assistant at the University of Oxford and an NIH Oxford-Cambridge Scholar and NIH Undergraduate Scholar at the National Institutes of Health. In 2013, they were a Co-Founder and CEO of Piper, Inc. and a Doctoral Fellow at the University of Oxford. In 2019, they became a Co-Founder and Board Director of Learn With Mochi. Finally, in 2021 and 2022, they became a Partner at NZVC and an Advisor at xRobotics, respectively.

Mark Pavlyukovskyy completed their Bachelor's degree in Biochemistry and Molecular Biology from Princeton University in 2013. Mark then went on to pursue a DPhil in Biomedical/Medical Engineering at the University of Oxford, which they completed in 2014. Before that, they attended the Texas Academy of Mathematics & Science from 2007 to 2009.