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Ashley Hotard Mattei has a diverse work experience, starting from 2015 when they joined Riverlands Insurance Services, Inc. as a Senior Executive Assistant. Ashley worked in this role for four years until 2019. In 2020, Ashley joined LCMC Health and held the position of Executive Coordinator of Finance from January 2020 to February 2022. Later, in October 2021, they transitioned to the role of Executive Coordinator of Diversity & Social Responsibility at LCMC Health.

Ashley Hotard Mattei has a background in sociology and attended the University of Nevada-Las Vegas, although specific dates for this education are not provided. In terms of additional certifications, Ashley has obtained a Psychological First Aid certification in February 2023 from the University of Minnesota. Additionally, they have certifications in Certified Fitness Trainer from the International Sports Sciences Association (ISSA), a Diversity and Inclusion Certificate from Cornell University, and is also a First Aid/CPR/AED Instructor certified by the National Safety Council. However, the obtained month and year for the latter certifications are not specified.