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Rutusha Gupta has a diverse work experience spanning multiple industries and roles. Rutusha started their career as a Business Development Executive at Quadge.com from July 2020 to September 2020. In 2021, they worked as a Content Writer for High Quality Assignment Help from March to April. Rutusha then joined PanGenomics International Pvt Ltd as a Student Trainee, where they gained hands-on experience in operating various gene-related testing techniques from April to June 2021.

In 2022, Rutusha interned at The Gujarat Cancer and Research Institute as a Summer Intern from May to August. Rutusha also worked as a Project Intern at Nirma University, where they contributed to a research project from June to July.

Currently, Rutusha is serving as a Research Associate at Lambda Therapeutic Research since May 2023.

Rutusha Gupta completed their Msc degree in Biotechnology from Nirma University in Ahmedabad, Gujarat, India, from 2021 to 2023. Prior to that, they pursued life sciences at the Institute of Advanced Research in Gandhinagar from 2018 to 2021. In their earlier education, Rutusha attended CU Shah High School for their 11th and 12th grades and St Anns High School (SSC) for their 10th grade, although the specific years were not provided. Additionally, they obtained certifications in "The Fundamentals of Digital Marketing" from Google Digital Unlocked and "Introduction to Cellular and Molecular Biology" from Saylor Academy, but the exact dates of completion were not mentioned.