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Estelle Thiot has a diverse work experience in the fields of clinical operations, research, and analytical chemistry. Estelle started their career in 2016 by completing a student internship at the Institut des Sciences Analytiques, where they gained practical knowledge in the field. In the following year, Estelle completed another student internship at the Laboratoire Pharma-dev, where they focused on the isolation and identification of secondary metabolites from a fungal strain using various separation techniques. Estelle then continued their academic journey with a student internship at the Laboratoire SPCMIB in 2017, where they conducted metabolomic studies using proton nuclear magnetic resonance (NMR) for the analysis of hypophosphatasia and acute renal insufficiencies. Estelle further expanded their skills by working as an analytical chemistry engineer at the Institut national de la recherche agronomique (INRA) in 2018, where they conducted NMR analysis and performed statistical analysis on metabolomic data. Estelle then transitioned to the clinical research field and worked as a clinical research associate at INSTITUT CLAUDIUS REGAUD from 2019 to 2020, and later as a clinical operations project manager assistant at Kayentis from 2022.

Estelle Thiot's education history begins with their enrollment at the Université Grenoble Alpes, where they pursued a Bachelor's degree in Chemistry and successfully obtained their Licence in 2015. Estelle proceeded to further their education at the same university, enrolling in the Master 1 program in Sciences and Engineering of Medicines in 2016. However, the end year for this program is not specified. Subsequently, they pursued a Master 2 degree in Medicinal and Pharmaceutical Chemistry at the Université Grenoble Alpes, completing the program in 2017. Following this, Estelle Thiot joined the Formatis-Multihealth Group in 2019 to enhance their clinical research skills as an Attachée de recherche clinique.