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Nikoo Saber has worked in various roles since 2001. From 2001-2008, they were a Postdoctoral Scholar at Caltech, followed by a Research Fellow at the University of Toronto (2004-2005), a Postdoctoral Fellow at Huntington Medical Research Institutes (2003-2004), and a Staff Scientist at the California Institute of Technology (2008). From 2005-2006, they were a Research Associate/Fellow at Children's Hospital Los Angeles and a Consultant at Orqis Medical. In 2009, they became a Senior Project Manager at The Hospital for Sick Children in Toronto. From 2014-2017, they were a Co-Founder at The Social Collective, and from 2015-present, they have been a Self-Employed Consultant providing technical and managerial expertise on various projects incorporating design, modeling, prototyping and fabrication via 3D printing.

Nikoo Saber has a PhD and MSc in Mechanical Engineering from Imperial College London.