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Madhukar Phule began their career as an Assistant Research Manager (F&B, Agri) at Dish Food & Events, LLC in 2010. In 2012, they joined Food Sciences Corporation as a Senior Research Manager (F&B), where they developed and implemented research and development procedures and techniques, and helped senior management and internal clients to understand the results and implications of research and development initiatives. Madhukar also prepared and managed research and development budget. In 2016, they founded Food & Beverage Innovation and Food & Beverage Innovations, and served as the Chief Executive Officer for both organizations.

Madhukar Phule obtained a Bachelor's degree in Food Technology and Processing from Mumbai University Mumbai between 2004 and 2008. Madhukar then went on to pursue a Master's degree in Food Science and Technology at the Mumbai Institute Of Management and Research between 2008 and 2010.