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Kathleen Ruegsegger began their work experience in 1999, at Thermo Fisher Scientific, where they held a leadership position in operational and supply chain. In 2015, they transitioned to Pyxis Solutions LLC, where they served as the Principal. In 2018, they joined Clinical Reference Laboratory as the COO. Most recently, in 2020, they have taken on the role of President at InnovaPrep LLC.

Kathleen Ruegsegger's education history includes a Bachelor's degree in Bacteriology from the University of Wisconsin-Madison. Kathleen also holds a Master's degree in Business Administration from Keller Graduate School. In addition, they have obtained a Certificate in Project Management from Keller Graduate School. Kathleen has also received a Master's Certificate in Project Management, but the institution and the specific month and year of obtaining this certification are not specified.