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Betsy Chestnut started their work experience in 1996 at Red Valve Company, Inc. where they held the position of Accounting and HR Manager until 2001. In 2001, they joined Industrial Scientific Corporation as the Global Corporate Controller.

Betsy Chestnut completed their Bachelor of Science degree in Accounting at La Roche University from 1992 to 1996. Following that, they pursued a Masters degree in Taxation from Robert Morris University, which they successfully completed from 1993 to 1996.