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Margaret MacDonald has held various roles in the healthcare and clinical research industries since 2008. Margaret began their career as a Microbiology Technician at Silliker Laboratories (now part of Méríeux NutriSciences) in 2008, followed by a role as a Clinical Chemistry Technician at Catch Incorporated (now part of Axis-Shield) in 2009. In 2010, they joined NanoString Technologies as a Clinical Research Associate and Research Associate. Margaret then moved to Kona Medical as a Clinical Research Associate in 2015, followed by a role as a Clinical Research Associate at C3 Research Associates (now part of Avance Clinical) in 2016. In 2017, they became a Protocol Lead at Seagen, followed by a role as a Clinical Trial Manager at Chinook Therapeutics, Inc. in 2021. Most recently, they joined Icosavax, Inc. in 2022 as a Clinical Operations Manager.

Margaret MacDonald obtained their Bachelor of Science in Neuroscience from Cedar Crest College between 2004 and 2008. In October 2015, they earned their Certified Clinical Research Associate (CCRA\u00ae) certification from the Association of Clinical Research Professionals.