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Marlies Leutfeld's work experience began in 1989 as a Student Assistant at the Fraunhofer Institut für Umweltchemie und Ökotoxikologie. Marlies then worked as a Typist at Annonce Verlag KG from 1990 to 1993, followed by a role as a Coordinator Typist Office at Duales System Deutschland GmbH from 1993 to 1994. Marlies then worked as a Manager at Heinrich Leutfeld GmbH from 1994 to 1995, and as an Administrator Freelancer at ZFMK Bonn from 1995 to 1998. Marlies took a break from their career for maternity leave from 1998 to 1999. Upon their return, they worked as an External Software Tester and a Clinical Project Assistant at DATATRAK International from 1999 to 2000. Marlies then became a User Support Specialist and a Senior User Support Specialist at the same company from 2000 to 2003. Marlies was later promoted to Solution Center Supervisor from 2003 to 2008. Marlies joined DATATRAK Deutschland GmbH in 2007 as an Assistant Director Business Operations Europe, and then moved to Hype Softwaretechnik GmbH in 2008. At Hype Softwaretechnik GmbH, they served as a Consultant from 2008 to 2010, Head of Custom Solutions from 2010 to 2011, and currently holds the role of Head of Professional Services Bonn, which began in 2011.

Marlies Leutfeld attended the Amplonius Gymnasium Rheinberg for an unspecified period of time. Later, from 1985 to 1992, they pursued a degree in Biology at The University of Bonn, where they obtained a Diplom.