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Margaret O'Neill's work experience includes roles at Harlem Lacrosse and T3 Lacrosse. At Harlem Lacrosse, they started as a Program Director in August 2019 before being promoted to Senior Program Director in September 2022. Their responsibilities in these roles are not specified. At T3 Lacrosse, they worked as an Operations Intern from May 2018 to August 2018. In this position, they were involved in preplanning for sporting events, provided on-site operational support during events, and coordinated with a local foundation to collect donations.

Margaret O'Neill completed their Bachelor of Arts degree in Sociology from Villanova University, where they attended from 2015 to 2019. In 2017, they briefly studied at John Cabot University, although the specific degree and field of study during their time there are not provided.