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Denise Delfino has a diverse work experience spanning over several industries. Denise is currently employed as a Clinical Trials Analyst at Guardian Research Network since January 2021. Prior to this role, they worked as a Neonatal Intensive Care Nurse at the University of Iowa Hospitals and Clinics from August 2006 to May 2020, and at WakeMed Hospital from August 2019 to March 2020. Denise also served as a Shift Supervisor in the Neonatal Intensive Care department at Mercy Medical Center in an unspecified time in 2013, and as a Manager of Marketing Operations at Assured Health Systems from 1992 to 1993. Prior to that, they worked as a Marketing Communications Manager at Preferred Health Care from 1989 to 1992. Their earliest recorded role was as a Database Administrator at Videolog from 1987 to 1989.

Denise Delfino holds a Bachelor's Degree in Registered Nursing/Registered Nurse, which they obtained from Coe College between the years 2002 and 2006. Prior to this, they pursued a Bachelor's Degree in History from the College of the Holy Cross, completing their studies from 1980 to 1984. In addition, they hold a Master's Degree in Communication and Media Studies from Fairfield University, having attended from 1985 to 1987.