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AJ Mafolasire has a diverse work experience. In 2005, they started their career as a Test Engineer at Caterpillar Inc., where they worked until 2007. In 2008, they joined Cummins Inc. as a Diesel Test Engineer and stayed until 2010. In 2016, they became a Resident Physician at Beth Israel Deaconess Medical Center, where they served until 2021. In 2019, AJ Mafolasire took on the role of Head of Data Science & Philanthropic Research at Givelify. Additionally, they also held the title of MD at Yale New Haven Hospital from an unknown start date until October 2021.

AJ Mafolasire has a diverse education history. They obtained a Doctor of Medicine (MD) degree from Yale University School of Medicine. Prior to that, AJ pursued their Master's degree in Mechanical Engineering at Purdue University. They also completed two Bachelor's degrees at Purdue University, one in Mechanical Engineering & Economics and another in Mathematics. Additionally, AJ earned a Master's degree in Health Services/Allied Health/Health Sciences, General from Yale University School of Medicine.