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Dianne O'Connell began their work experience in 1994 at Quincy Medical Center, where they worked as a Certified Surgical Technologist until 2000 and then transitioned to a Registered Nurse in 2000-2001. In 2010, they joined Frisbie Memorial Hospital as the Chairperson of the Go Green team. Later in 2012, they became the Director Surgical Services.

Dianne O'Connell obtained an MBA from Daniel Webster College from 2006 to 2008. Prior to that, they completed their Associates degree in Nursing at Quincy College from 1998 to 2000.