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Nils Knofius has a range of work experience starting in 2008 as a Trainee at Prinovis Ltd. & Co. KG. Nils then worked as a Graduate Student at ASML in 2014 before moving on to become a PhD Candidate at the University of Twente from 2014 to 2018. Nils continued their work at the University of Twente as a Postdoctoral Researcher from 2019 to 2020. In 2019, they also became the Owner of AMQ Services B.V. Their most recent role is Head Of Digitalization at Fieldmade AS, where they started in 2020.

Nils Knofius completed their education starting from 2008 to 2012, where they obtained a Bachelor of Science (B.Sc.) degree in Industrial Engineering and Business Administration from Fachhochschule Nordakademie Elmshorn. Following that, they pursued a Master of Science (M.Sc.) degree in Industrial Engineering and Management from the University of Twente from 2012 to 2014. During their master's program, they also attended National Chiao Tung University as an exchange student, focusing on Industrial Engineering and Management in 2013-2014. Finally, from 2014 to 2018, Nils Knofius pursued a Doctorate degree in Industrial Engineering at the University of Twente, ultimately obtaining a Doctor of Philosophy (PhD).