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Louise Pequignot has been working in the animation industry since 2010. Louise began their career as an R&D Engineer Trainee at SHOOT THE BOSS, followed by R&D Engineer roles at Duboi and Mac Guff. In 2011, they transitioned to a Katana Pipeline TD role at Mikros Image, and in 2016, they moved to a similar role at Mikros Animation Montréal. In 2017, they became a Pipeline TD at ON Animation Studio Montreal, followed by Senior Pipeline TD roles at Double Negative and L'Atelier Animation Inc. in 2018 and 2020, respectively. Most recently, in 2021, they began working as a Senior Pipeline Developer at Felix & Paul Studios.

Louise Pequignot obtained their Higher National Certificate from UTBM in 2007, majoring in IT applications, Multimedia, Design. Louise then went on to earn their Master's degree from Université Paris-Est Marne-la-Vallée in 2010, specializing in Computer Graphics.